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human foxo 1  (Addgene inc)


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    Structured Review

    Addgene inc human foxo 1
    Human Foxo 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+foxo+1/1098+pcDNA+GFP+FKHR+(Plasmid+%239022)/pmc04835944-195-12-17
    Average 93 stars, based on 7 article reviews
    human foxo 1 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Construct:

    Article Title: Direct regulation of IGF-binding protein 1 promoter by interleukin-1β via an insulin- and FoxO-1-independent mechanism
    Article Snippet: When confluency reached 70–80%, cells were transfected as indicated with 1 μg of purified DNA using Lipofectamine 3000 reagent (Life Technologies, Carlsbad, CA) following the manufacturer's protocol. .. The following constructs were used for transfection: a green fluorescent protein (GFP)-tagged human FoxO-1 [1,098 pcDNA GFP-FKHR, Addgene plasmid no. 9022, gift from William Sellers ( 42 )], wild-type IGFBP1 promoter [Igfbp1 promoter/pGL3, Addgene plasmid no. 12146, a gift from Domenico Accili ( 41 )], pRL-TK plasmid expressing the Renilla reporter (gift from Dr. Karyn Esser), siRNAi against FoxO-1 (siRNA ID no. s136655; Ambion, Life Technologies), or ΔIRE-IGFBP1 promoter. ..

    Transfection:

    Article Title: Direct regulation of IGF-binding protein 1 promoter by interleukin-1β via an insulin- and FoxO-1-independent mechanism
    Article Snippet: When confluency reached 70–80%, cells were transfected as indicated with 1 μg of purified DNA using Lipofectamine 3000 reagent (Life Technologies, Carlsbad, CA) following the manufacturer's protocol. .. The following constructs were used for transfection: a green fluorescent protein (GFP)-tagged human FoxO-1 [1,098 pcDNA GFP-FKHR, Addgene plasmid no. 9022, gift from William Sellers ( 42 )], wild-type IGFBP1 promoter [Igfbp1 promoter/pGL3, Addgene plasmid no. 12146, a gift from Domenico Accili ( 41 )], pRL-TK plasmid expressing the Renilla reporter (gift from Dr. Karyn Esser), siRNAi against FoxO-1 (siRNA ID no. s136655; Ambion, Life Technologies), or ΔIRE-IGFBP1 promoter. ..

    Plasmid Preparation:

    Article Title: Direct regulation of IGF-binding protein 1 promoter by interleukin-1β via an insulin- and FoxO-1-independent mechanism
    Article Snippet: When confluency reached 70–80%, cells were transfected as indicated with 1 μg of purified DNA using Lipofectamine 3000 reagent (Life Technologies, Carlsbad, CA) following the manufacturer's protocol. .. The following constructs were used for transfection: a green fluorescent protein (GFP)-tagged human FoxO-1 [1,098 pcDNA GFP-FKHR, Addgene plasmid no. 9022, gift from William Sellers ( 42 )], wild-type IGFBP1 promoter [Igfbp1 promoter/pGL3, Addgene plasmid no. 12146, a gift from Domenico Accili ( 41 )], pRL-TK plasmid expressing the Renilla reporter (gift from Dr. Karyn Esser), siRNAi against FoxO-1 (siRNA ID no. s136655; Ambion, Life Technologies), or ΔIRE-IGFBP1 promoter. ..

    Expressing:

    Article Title: Direct regulation of IGF-binding protein 1 promoter by interleukin-1β via an insulin- and FoxO-1-independent mechanism
    Article Snippet: When confluency reached 70–80%, cells were transfected as indicated with 1 μg of purified DNA using Lipofectamine 3000 reagent (Life Technologies, Carlsbad, CA) following the manufacturer's protocol. .. The following constructs were used for transfection: a green fluorescent protein (GFP)-tagged human FoxO-1 [1,098 pcDNA GFP-FKHR, Addgene plasmid no. 9022, gift from William Sellers ( 42 )], wild-type IGFBP1 promoter [Igfbp1 promoter/pGL3, Addgene plasmid no. 12146, a gift from Domenico Accili ( 41 )], pRL-TK plasmid expressing the Renilla reporter (gift from Dr. Karyn Esser), siRNAi against FoxO-1 (siRNA ID no. s136655; Ambion, Life Technologies), or ΔIRE-IGFBP1 promoter. ..



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